Engineering photoautotrophic microorganisms to directly convert carbon dioxide into platform chemicals is an attractive approach for chemical sustainability and carbon mitigation. Here, an engineered cyanobacterium Synechococcus elongatus PCC 7942 was developed to produce succinic acid directly from ambient carbon dioxide. Inhibition of succinate dehydrogenase and glycogen synthase by CRIPSR interference increased carbon flux towards succinic acid. Dual inhibition of these two genes led to an 82% increase in titer. The resulting strain produced 4.8 g/L of succinic acid in a 28-days cultivation. However, cells after the 28-days cultivation became non-viable and cannot continue production. This issue was addressed by re-inoculation with fresh cells into the production medium. This strategy enabled continuous succinic acid accumulation, reaching a final titer of 8.9 g/L. This study provides a sustainable route to succinic acid directly from carbon dioxide and a potential method to overcome the low titer limitation of cyanobacterial-based bioproduction for practical applications.
精誠集團攜手陽明交大深化產學合作,捐贈價值7千萬元的GARAOTUS雲平台與軟體服務,提供陽明交大生物科技學院全台第一套的GAaaS(Genomics Analytics as a Service)國際級系統架構與專業服務,建立世界級基因分析實驗室所使用的軟硬體技術與配備,幫助提升研究效能與成果整合;並於生科院開立工程與計算生醫產業碩士產學專班,投入教學與實做演練,培育跨領域國際級生醫人才。
GARAOTUS負責人薛聖傑表示:「生物技術研究的計算量龐大,這些資料往往儲存個別實驗室或研究單位,不利於彼此間合作開發。GARAOTUS的平台架構即是專為大量資料快速運算所設計,透過GAaaS(Genomics Analytics as a Service)國際級系統架構與專業服務,可以協助研究人員透過雲平台快速整合散落各地的研究資料,並且快速運算,對於新藥或是精準醫療相關的發展有很大的助益。GARAOTUS 雲服務平台的醫療行業解決方案,目前已在香港導入,未來也將積極針對新加坡、印度等東南亞市場推廣。」