林志生教授研究團隊發表研究成果於Biosensors & Bioelectronics
連結網址： https://pubmed.ncbi.nlm.nih.gov/39102773/
Abstract
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is
facilitated by its trimeric surface spike protein, which binds to the human angiotensin-
converting enzyme 2 (hACE2) receptor. This critical interaction facilitates viral entry
and is a primary target for therapeutic intervention against COVID-19. However, it is
difficult to fully optimize viral infection using existing protein-protein interaction
methods. Herein, we introduce a nano-luciferase binary technology (NanoBiT)-based
pseudoviral sensor designed to stimulate the dynamics of viral infection in both living
cells and animals. Infection progression can be dynamically visualized via a rapid
increase in luminescence within 3 h using an in vivo imaging system (IVIS).
Inhibition of viral infection by baicalein and baicalin was evaluated using a NanoBiT-
based pseudoviral sensor. These results indicate that the inhibitory efficacy of
baicalein was strengthened by targeting the spike protein, whereas baicalin targeted
the hACE2 protein. Additionally, under optimized conditions, baicalein and baicalin
provided a synergistic combination to inhibit pseudoviral infection. Live
bioluminescence imaging was used to evaluate the in vivo effects of baicalein and
baicalin treatment on LgBiT-hACE2 mice infected with the BA.2-SmBiT spike
pseudovirus. This innovative bioluminescent system functions as a sensitive and
early-stage quantitative viral transduction in vitro and in vivo. This platform provides
novel opportunities for studying the molecular biology of animal models.
